Nanded Fata, Pune, Maharashtra 411041
Unitech Research Solutions
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Knowledge Repository

Scientific Resources & Knowledge Hub

Curated protocol guides, technical insights, case studies, and answers to common preclinical and analytical testing questions.

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Technical Insights

Research Articles & SOP Insights

Practical advice from our bench scientists on standardizing assays, troubleshooting chromatography, and writing Scopus-indexed manuscripts.

Chromatography SOP

Optimizing HPLC Method Validation as per ICH Q2(R1)

A step-by-step walkthrough on establishing specificity, linearity, limit of detection (LOD), limit of quantitation (LOQ), and precision for novel pharmaceutical formulations.

Updated: 2026 | Technical Dossier
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Cell Biology

MTT vs. LDH Cytotoxicity: Selecting the Right Assay

Comparing mitochondrial dehydrogenase activity (MTT) against lactate dehydrogenase (LDH) membrane leakage to prevent false positives with phytoconstituents.

Updated: 2026 | Protocol Guide
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Preclinical Models

Histological Scoring of Carbon Tetrachloride Liver Injury

Standardizing Ishak and Metavir scoring systems for steatosis, ballooning degeneration, and collagen deposition with Masson's Trichrome staining.

Updated: 2026 | Pathology Note
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Scholar Toolkits

Research Guides for M.Pharm & Ph.D. Scholars

Practical frameworks to accelerate experimental design, sample size power calculations, and thesis defense preparation.

Statistical Power & Group Sizing in Animal Studies

Resource Guide #01

Guidance on applying Resource Equation vs. Power Analysis to calculate ethically acceptable sample numbers (n=6 per group) that satisfy university Institutional Animal Ethics Committees (IAEC).

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Sample Preparation SOP for HPLC & Mass Spec

Resource Guide #02

Standardized protocols for biological fluid deproteinization, solid-phase extraction, 0.22 µm syringe filtration, and autosampler vial labeling to prevent column fouling.

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Answers

Frequently Asked Questions

Clear answers regarding sample shipment, testing turnaround times, confidentiality, and regulatory reporting.

What is the typical turnaround time for a testing study?
Routine analytical testing (HPLC / HPTLC assays, UV-Vis, phytochemical profiling) is typically completed within 5 to 10 business days. Standard in-vitro cytotoxicity (MTT assays) takes approximately 7 to 12 business days. Comprehensive in-vivo animal disease studies with histopathology and multi-cytokine ELISA take between 3 to 8 weeks depending on the dosing duration.
How are proprietary molecules and client intellectual property safeguarded?
Prior to discussing specific chemical structures or receiving physical samples, Unitech Research Solutions executes a comprehensive, legally binding Non-Disclosure Agreement (NDA). All data, chromatograms, and biological results remain 100% the intellectual property of the sponsoring client or scholar.
How should biological or chemical samples be shipped to your Pune lab?
Solid botanical extracts and chemical powders can be shipped at ambient temperature via courier in sealed amber vials. Thermolabile proteins, cell lysates, or tissue biopsies should be shipped on dry ice or with frozen gel packs accompanied by a Material Safety Data Sheet (MSDS) and our Sample Submission Form.
Can URS help with university thesis documentation and Scopus publication?
Yes. For academic scholars (M.Pharm, Pharm.D, Ph.D.), we provide complete statistical analyses (ANOVA, Dunnett's, p-value calculations), high-resolution calibrated photomicrographs with scale bars, GraphPad Prism vector graphs, and comprehensive technical reports ready to be integrated into dissertations and peer-reviewed journal manuscripts.
What is the operational status of RT-PCR / qPCR and advanced genomic assays?
Real-Time PCR (qPCR) and advanced genomic quantitation assays are scheduled operationally based on project batch requirements and primer validation. Contact our laboratory coordinator with your target gene panel to confirm current run dates.
Do you offer customized research protocols for unique drug targets?
Absolutely. Our senior scientists specialize in tailoring multi-parameter research protocols combining molecular docking, botanical extraction, HPLC standardization, in-vitro cytotoxicity, and customized animal models tailored to your specific therapeutic hypothesis.
Latest News

Laboratory Updates & Academic Events

Stay informed on upcoming workshops, new instrument calibrations, and research highlights.

Workshop Announcement

5-Day Hands-on Workshop on HPLC & HPTLC

Registrations open for the upcoming cohort focusing on reverse-phase method validation as per ICH guidelines in our Pune analytical suite.

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Facility Upgrade

Expansion of In-Vitro Apoptosis Assay Suite

URS has added multi-caspase activation and rhodamine-123 mitochondrial membrane potential screening to its routine in-vitro oncology testing menu.

Explore Assay Menu
Academic Partnership

New Academic MoU Partnerships Signed

Formal training and preclinical research MoUs finalized with leading pharmacy colleges in Maharashtra to support doctoral research scholars.

Learn About Institutional MoUs