Validated experimental models simulating human disease pathophysiology for screening synthetic compounds, phytopharmaceuticals, and novel drug delivery systems.
Standardized preclinical models of Type-1, Type-2 diabetes mellitus, insulin resistance, and diabetic complications.
• Streptozotocin (STZ) Induced Diabetes (Single High Dose & Multi-Low Dose)
• Alloxan Induced Diabetes
• High-Fat Diet (HFD) + Low Dose STZ (Type 2 Metabolic Syndrome)
• Oral Glucose Tolerance Test (OGTT) & Insulin Tolerance Test (ITT)
Serum Glucose, Glycated Hemoglobin (HbA1c), Insulin levels, Lipid Profile (TC, TG, HDL, LDL), Pancreatic Islet of Langerhans beta-cell histology with H&E and aldehyde fuchsin staining.
In-vitro cancer cell line panel screening and tumor regression studies evaluating antiproliferative pathways and apoptosis.
• Human Breast Cancer (MCF-7, MDA-MB-231)
• Human Cervical Cancer (HeLa)
• Human Lung Carcinoma (A549)
• Colon & Liver Cell Lines (HT-29, HepG2)
• In-Vitro MTT Cytotoxicity, IC50 Curve, Clonogenic Assays
Annexin V-FITC / PI dual staining, Caspase-3/7 activity, Mitochondrial Membrane Potential (JC-1), Intracellular ROS, and DAPI nuclear chromatin condensation.
Acute and chronic inflammatory models, rheumatoid arthritis models, and systemic cytokine profiling.
• Carrageenan Induced Rat Paw Edema (Acute Inflammation)
• Freund's Complete Adjuvant (FCA) Induced Arthritis (Chronic Autoimmune)
• Cotton Pellet Induced Granuloma (Sub-acute Proliferative)
• Formalin Induced Paw Licking & Edema
Plethysmometer paw volume tracking, Myeloperoxidase (MPO), Pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), Joint histopathology (synovial hyperplasia, pannus, cartilage erosion).
Kidney stone formation (nephrolithiasis), acute kidney injury, and renal tubular protection models.
• Ethylene Glycol + Ammonium Chloride Induced Urolithiasis
• Cisplatin / Gentamicin Induced Nephrotoxicity
• In-Vitro Calcium Oxalate Nucleation & Aggregation Turbidimetric Assay
Urinary Volume, Calcium, Oxalate, Phosphate, Serum Creatinine, Blood Urea Nitrogen (BUN), Uric Acid, Kidney crystal deposit scoring under polarized microscopy and H&E.
Preclinical evaluation of neuroprotective agents, cognitive enhancers, anxiolytics, and antidepressants.
• Scopolamine Induced Dementia / Memory Impairment (Morris Water Maze, Elevated Plus Maze)
• Forced Swim Test (FST) & Tail Suspension Test (TST) for Depression
• Open Field Test & Light-Dark Box for Anxiolytic Screening
• Haloperidol Induced Catalepsy (Parkinsonian Rigidity)
Brain Acetylcholinesterase (AChE) Activity, Brain Lipid Peroxidation (MDA), Superoxide Dismutase (SOD), Catalase, Brain tissue H&E and Nissl staining.
Assessment of topical herbal formulations, hydrogels, and nanofiber dressings on cutaneous wound repair.
• Excision Wound Model (Rate of Contraction & Epithelialization Period)
• Incision Wound Model (Tensiometer Breaking Strength)
• Burn Wound Model (Partial-Thickness Thermal Injury)
• Diabetic Wound Healing (STZ Excision Model)
Skin Hydroxyproline (Collagen Synthesis), Hexosamine, Total Protein, Masson's Trichrome collagen deposition scoring, and re-epithelialization histology.
Phytochemical identification, fingerprinting, and standardized bioactivity evaluation of ethnomedicinal plants.
• Plant Authentication & Voucher Specimen Herbarium
• Maceration, Soxhlation, Microwave & Ultrasound Extraction
• Column Fractionation & Marker Isolation
• High-Performance Thin-Layer Chromatography (HPTLC) Fingerprints
DPPH Free Radical Scavenging, ABTS, Nitric Oxide, Alpha-Amylase & Alpha-Glucosidase inhibition, Anti-lipase assays.
Engineering novel colloidal carriers, polymeric matrices, and lipid formulations to enhance bioavailability.
• Solid Lipid Nanoparticles (SLNs) & Nanostructured Lipid Carriers (NLCs)
• Self-Emulsifying Drug Delivery Systems (SEDDS / SNEDDS)
• Polymeric Nanoparticles, Liposomes & Niosomes
• Thermoreversible & Bioadhesive In-Situ Hydrogels
Entrapment Efficiency (%EE), In-Vitro Dialysis Membrane Drug Release, Korsmeyer-Peppas Release Kinetics, and ICH Accelerated Stability.
